ZFIQ

1. Introduction

The software aims to provide a set of practical and effective image analysis tools for quantitative, reproducible and accurate interpretation of zebrafish imaging data, in particular, for streamlined processing and analysis of bioimaging data.This Quick Start Guide to the ZFIQ shows how to build the pipeline. For a more detailed description of Pipeline features, please see the ZFIQ-Help.

2. Installation

System Requirement :

Windows 2000, Windows XP and Windows Vista

Compile Environment (Optional):

MinGW (included in our package)

Microsoft Visual Studio 6, 7, 8

Step 1: Run setup.exe
Step 2: Then follow the step by step installation
Step 3: Reboot you computer after installation

* Possible problem in Installation:
If the paths are not added to system environment or users want to use User Environment instead of System Environment, users can add these 3 paths to Path manually:
1. {app}\MinGW\bin
2. {app}\bin
3. {app}\dll
Where {app} is the installation path.

3. Interface overview

There are 4 windows in the GUI, that is:

Filter Database Window: the window on the very left, it contains all the OpenCV functions and functions defined by users.

Pipeline Construction Window: the window in the center of the GUI. This is the place you can construct a pipeline using a mouse. Please see How to construct a pipeline for details.

Execution Sequence Window: the window on the top right. This window is a list of function names used in the pipeline. The order or sequence of functions in the list is the order in which the functions will be executed.

Parameter Editing Window: the window on the bottom right. Click a Pipeline Node in the pipeline, you would find the property of input and output parameters of the node. One can edit the value of a certain parameter. For details, please see How to edit parameters.

4. Building a pipeline

1.) Drag filters
2.) Drop filters
3.) Connect filters
4.) Adjust parameters in the filters (optional)
5.) Adjust execute orders (optional)

For details, please see the Video of Pipeline Construction and Usage .

5. Execution

1.) Save constructed pipeline or load existing pipeline
2.) Use “Build All (F9)” to generate the source code and executable file.
3.) Use “Run with parameters (F11)” to do single image processing.
4.) Use “Run Batch File” to do batch images processing

For details, please see the Video of Pipeline Construction and Usage .

Click the here to see large image.